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pet21(+) expression vector  (Merck & Co)

 
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    Merck & Co pet21(+) expression vector
    A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
    Pet21(+) Expression Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet21%28+%29+expression+vector/bio_rxiv__2024__12__02__626473-51-5-6?v=Merck+%26+Co
    Average 90 stars, based on 1 article reviews
    pet21(+) expression vector - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Rescue of bacterial motility using two and three-species FliC chimeras"

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    Journal: bioRxiv

    doi: 10.1101/2024.12.02.626473

    A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
    Figure Legend Snippet: A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

    Techniques Used: Incubation

    SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.
    Figure Legend Snippet: SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

    Techniques Used: SDS Page, Construct, Plasmid Preparation, Positive Control, Marker, Control


    Figure Legend Snippet:

    Techniques Used:



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    Image Search Results


    A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

    Journal: bioRxiv

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    doi: 10.1101/2024.12.02.626473

    Figure Lengend Snippet: A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

    Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

    Techniques: Incubation

    SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

    Journal: bioRxiv

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    doi: 10.1101/2024.12.02.626473

    Figure Lengend Snippet: SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

    Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

    Techniques: SDS Page, Construct, Plasmid Preparation, Positive Control, Marker, Control

    Journal: bioRxiv

    Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

    doi: 10.1101/2024.12.02.626473

    Figure Lengend Snippet:

    Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

    Techniques: